Abstract
As a processing by-product, green pea hull (GPH) was found to be rich in phenolic components in our previous studies. In this study, UHPLC-LTQ-OrbiTrap-MS (Ultra performance liquid chromatography-linear ion trap orbitrap tandem mass spectrometry) technique was used to quantify polyphenols, and DSS (sodium dextran sulfate)-induced colitis mouse model was established to explore the effect of GPH extracts on colitis. The results showed that quercetin and its derivatives, kaempferol trihexanside and catechin and its derivatives were the main phenolic substances in the extract, reaching 2836.57, 1482.00 and 1339.91 µg quercetin/g GPH extract, respectively; GPH extracts can improved inflammatory status, repaired colonic function, regulated inflammatory factors, and restored oxidative balance in mice. Further, GPH extracts can activate Keap1-Nrf2-ARE signaling pathway, regulate downstream antioxidant protease and gut microbiota by increasing F/B value and promoting the growth of Lactobacillaceae and Lachnospiraceae, and improve the level of SCFAs (short-chain fatty acids) to relieve DSS-induced colitis in mice. Therefore, GPH may be a promising dietary resource for the treatment of ulcerative colitis.
Highlights
Lesjak et al [30] studied different quercetin and its derivatives and found that they all had good antioxidant and anti-inflammatory activities, and they were present in the systemic circulation, which suggested that bioavailability and its metabolites should be taken into account when defining the activity of active substances
The results indicated that the ameliorative effect of green pea hull (GPH) extracts on DSS-induced colitis in mice may be related to the maintenance of inflammatory cytokine balance
Our results suggest that way, regulating gut microbiota, and increasing the level of
Summary
Ulcerative colitis (UC), a type of inflammatory bowel disease (IBD), is a chronic intestinal inflammation disease with symptoms of abdominal pain, cramps, bloating, diarrhea, constipation and/or blood defecate [1,2]. UC causes a significant decline in the quality of life of patients and is a burden on the medical and health system due to its increasingly higher morbidity, especially in younger patients [1,2,3]. Several studies have shown that IBD is associated with genetic factors, lifestyle, environment, immune dysfunction, and the intestinal ecosystem, the exact causes have not been definitively confirmed [4,5]. Aminosalicylate, sulfasalazine, mesalazine, glucocorticoids and immunosuppressants are commonly used drugs to treat UC, but long-term use may cause fever, vomiting, acute pancreatitis and other side effects [6,7]. Diet has been suggested as a treatment for IBD due to the natural active substances in food, such as polyphenols [1,5]
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