Abstract
Aim:Treatment of inflammatory disorders relies on the intervention in immune responses thereby restoring homeostasis. IL-10 is a cytokine with therapeutic potential, but until now has not been as successful as previously anticipated. A reason for this may be that IL-10 responsiveness depends on the environment of the inflamed tissue. In this study we investigated whether GM-CSF is able to influence IL-10-mediated responses.Methodology:Dendritic cells and macrophages were differentiated from mouse bone marrow and treated or depleted from GM-CSF prior to analyze their response to IL-10. Activity was assessed by measuring cytokine expression upon lipopolysaccharide stimulation, IL-10-induced signaling and down-stream gene expression.Conclusion:This study describes that GM-CSF negatively regulates IL-10-mediated responses.
Highlights
The local inflammatory cytokine environment might affect the response of immune cells toward IL-10
We observed that GM-CSF-differentiated dendritic cells from mouse bone marrow respond differently toward IL-10 than bone marrow-derived macrophages
Treatment of macrophages with GM-CSF negatively affects their responses toward IL-10
Summary
Treatment of inflammatory disorders relies on the intervention in immune responses thereby restoring homeostasis
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