Gold Immunochromatographic Assay for Rapid On-Site Detection of Lincosamide Residues in Milk, Egg, Beef, and Honey Samples.

Abstract

Lincosamides (LMs), include clindamycin (CLIN), lincomycin (LIN), and pirlimycin (PIR), that are widely used as veterinary drugs. LM residues in edible animal origin foods endanger human health and are in urgent need of establishing fast, simple, and highly sensitive detection methods. A gold immunochromatographic strip is prepared to detect CLIN, LIN, and PIR residues simultaneously with a single monoclonal antibody. This antibody is obtained with the design of a novel Hapten and can simultaneously recognize CLIN, LIN, and PIR. Under optimized conditions, the strip results can be semi-quantitatively evaluated with the naked eye within 15min, with cut-off values in phosphate-buffered saline of 1ngmL-1 for CLIN, 10ngmL-1 for LIN, and 25ngmL-1 for PIR, respectively. Besides, the strip can also be quantified using a hand-held strip scanner, and the spiked samples are used for establishing matrix curves. The limits of detection for CLIN, LIN, and PIR in spiked milk, egg, beef, and honey samples can satisfy the detection requirement. The utility of this strip is also confirmed by positive honey sample. In short, this strip should be expected to be a useful tool for the rapid on-site screening of lincosamide residues in milk, egg, beef, and honey samples.