Abstract
Glycosphingolipids containing neuraminic acid (gangliosides) have been isolated from two lines of mouse neoplastic mast cells. Thin-layer chromatography and chemical analysis of these lipids suggest the presence of at least three different types of gangliosides: N-acylneuraminosyl-monoglycosylceramide, N-acylneuraminosylmonaminoglycosyl-triglycosylceramide and N-acyl-dineuranminosyl-monoaminoglyco-syldiglycosylceramide. The intracellular distribution of the gangliosides differs from cerebroside sulfate in that the neuraminic acid lipids are primarily found in small-particle fractions, but the cerebroside sulfate occurs mainly in the amine containing large-particle fractions. Density gradient centrifugation of this large-particle fraction has demonstrated that the cerebroside sulfate is retained in a subfraction showing high fumarase activity, characteristic of mitochondria, and separable from the amine containing granules. The chemical analysis of purified mast cell cerebroside sulfate showed an approximately equimolar ratio of sphingosine, hexose and sulfate.
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