Abstract
The previous claims by others of a novel choline transfer reaction involving CDP-choline and leading to the conversion of [U- 14C]glycero-3-phosphate to glycero-3-phosphocholine in liver sub-cellular fractions could not be confirmed. Apparent labelling of glycero-3-phosphocholine, isolated by paper chromatography, was, in fact, explained by contamination by other radioactive metabolites, especially d-glucose. A synergistic effect on gluconeogenesis from [U- 14C]glycero-3-phosphate was obtained by incubating well-washed mitochondria with washed microsomes.
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