Abstract
This article contains data related to the researc.h article entitled “Yeast-derived ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM” by Cecílio et al. (2015) [1]. ArtinM, a D-mannose-binding lectin isolated from the seeds of Artocarpus heterophyllus, exerts immunomodulatory and regenerative activities through its Carbohydrate Recognition Domain (CRD) (Souza et al., 2013; Mariano et al., 2014 [2], [3]). The limited availability of the native lectin (n-ArtinM) led us to characterize a recombinant form of the protein, obtained by expression in Saccharomyces cerevisiae (y-ArtinM). We compared the carbohydrate-binding specificities of y-ArtinM and n-ArtinM by analyzing the binding of biotinylated preparations of the two lectin forms using a neoglycolipid (NGL)-based glycan microarray. Data showed that y-ArtinM mirrored the specificity exhibited by n-ArtinM.
Highlights
Data accessibilityGraphs and table The data were generated from a NGL-based microarray system [4]
Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM
This article contains data related to the researc.h article entitled “Yeast-derived ArtinM shares structure, carbohydrate recognition, and biological effects with native ArtinM” by Cecílio et al (2015) [1]
Summary
Graphs and table The data were generated from a NGL-based microarray system [4]. N-ArtinM and y-ArtinM forms were biotinylated and analyzed for binding using a NGL-based microarray (in-house designation ‘Array Sets 18–22bis’) containing 255 lipid-linked glycan probes (Table 1). The data derived from the NGL-based microarray analyses provide important information on the carbohydrate binding specificities of y-ArtinM and n-ArtinM, and serve as the basis for further studies on the fine specificities of the lectins using other microarray systems or complementary techniques. We analyzed the native form of ArtinM and its yeast-derived counterpart, in terms of their ability to bind to 255 glycans distributed in a microarray platform, in order to identify whether n-ArtinM and y-ArtinM shared sugar-recognition specificity. Y-ArtinM showed higher fluorescence intensity than n-ArtinM
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