Glutamate receptors in the olfactory bulb synaptic circuitry: heterogeneity and synaptic localization of N-methyl-d-aspartate receptor subunit 1 and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor subunit 1

Abstract

In this study, we analysed the molecular heterogeneity and synaptic localization of the N-methyl-d-aspartate receptor subunit 1 and the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit 1 in the olfactory bulb glomerular synaptic circuitry. Semiquantitative reverse transcriptase polymerase chain reaction showed that approximately 40% of the N-methyl-d-aspartate receptor subunit 1 messenger RNA splice variants contain the N1, exon which conveys specific functional properties on the channel. In other forebrain and hindbrain regions that we examined, the ratio of the N1-containing (receptor subunit 11XX) to N1-lacking (receptor subunit 10XX) N-methyl-d-aspartate receptor subunit 1 messenger RNAs varied considerably. The cellular and subcellular distribution of N-methyl-d-aspartate receptor subunit 1 and AMPA receptor subunit 1 was investigated with antibodies generated against the C-terminal domain of the individual subunits [Petralia R. S. et al. (1994) J. Neurosci.14, 667–696; Wenthold R. J. et al. (1992) J. biol. Chem. 267, 501–507]. Both N-methyl-d-aspartate receptor subunit 1 and AMPA receptor subunit 1 were localized to the postsynaptic density of asymmetric synapses established by olfactory receptor neuron terminals with the dendrites of mitral and tufted cells. Not all of these synapses, however, were labelled.These results are consistent with the notion that glutamate is the neurotransmitter at the olfactory nerve to mitral and tufted cell synapses, and suggest a high heterogeneity in the expression of the postsynaptic glutamate receptors.