Abstract
The use of good electro-active polyaniline at high pH for immobilizing glucose oxidase is reported. The response current increased with increasing potential from 0.35 to 0.70 V. The maximum response current occurred at about pH 7.0. The relationship between response current and the glucose concentration was linear from 0.005 to 10.0 mmol dm−3. The Michaelis–Menten constant Km', maximum response current imax and the apparent activation energy (Ea) were 31.59 mmol dm−3, 21.28 µA and 32.58 kJ mol−1, respectively. The response currents of the biosensor increased with increasing temperature. The biosensor was characterized by FTIR, UV-Vis spectra and AC impedance.
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