Glucose and temperature sensitive luminescence ZnCdS nanoparticles

Abstract

ABSTRACT The effect of glucose on fluorescence characteristics of synthesized ZnCdS nanoparticles in glucose oxidase solution has been investigated. It has been shown that, on addition of glucose, the nanoparticle fluorescence is quenched. Dynamics of fluorescence quenching with increasing glucose concentration was observed. Threshold sensitivity of recording of glucose concentration has been 0,25 mg/ml. So the presented effect opens the facilities for application of synthesized ZnCdS nanoparticles for the glucose control in bodyfluids. Keywords: glucose , glucose oxidase, hydrogen peroxide , luminescent semiconductor nanoparticle, spectroscopy, quenching 1. INTRODUCTION The quantitative determination of glucose in biological fluids is performed to diagnose diabetes mellitus, estimate its course and also to conduct treatment of diseases running with changing glucose concentration. At the present time to determine glucose concentration, a large number o f different techniques has been developed, which is bound up with the need to control glucose concentration in biological fluids, such as blood, at diagnosis and treatment of various diseases, first of all diabetes. Currently biosensors have broad applicat ions in medicine and biotechnology. They have high speed and sensitivity. Biosensors are safe to use, quite accurate; small in size and available for mass production. But there are still some problems, such as narrow linear range and stability. Biosensors are not very durable, and therefore require careful maintenance. There are difficulties in the use of biosensors, associated with low thermal stability. Any biosensor consists of two basic functional elements: sensitive biological element and physical tran sducer of signal ( transducer ), transforming the concentration signal into an electrical one. As a sensitive biological material use all types of biological structures: enzymes, antibodies, receptors, nucleic acids, and even living cells. The enzyme and cel l biosensors are most developed. All of sensitive biological elements can be combined with different transducers. Electrochemical converters (electrodes), various kinds of optical converters, gravitational, calorimetric and resonance systems can be transdu cers. This will create a large variety of different types of biosensors