Abstract
In the presence of stress, the hypothalamic-pituitary-adrenal (HPA) axis activity can be enhanced to promote the secretion of a large amount of glucocorticoids (GCs), which play an important role in the anabolism and catabolism of skeletal muscle. When the endogenous and exogenous glucocorticoids are deficient or excessive, the body will produce stress-related resistance and change the protein metabolism. In this study, we investigated the effect of GC receptor GRα on protein breakdown and synthesis in porcine skeletal muscle cells (PSCs). Overexpression of GRα was shown to increase the expression of protein degradation-related genes, while knockdown of GRα decreased the expression of these genes. Additionally, we found a relationship between GRα and solute carrier family 2 member 4 (SLC2A4), SLC2A4 expression level increases when stress occurs, suggesting that increasing SLC2A4 expression can partially alleviate stress-induced damage, and we found that there is a combination between them via luciferase reporter assays, which still needs to be confirmed in further studies.
Highlights
We investigated the impact of GRα on protein deposition-related genes in porcine skeletal muscle cells (PSCs) and explored the relationship between GRα and SLC2A4
Previous studies have shown that DEX affects the expression of protein depositionrelated genes in PSCs, so we further studied the effects of GRα on protein depositionrelated genes in PSCs by the overexpression and interference of GRα
There are a variety of stresses in the growth and development of livestock
Summary
Glucocorticoids (GCs) are a type of steroid hormones secreted by the adrenal cortical bundle, and their secretion could be increased under stress [1]. The feedback regulation of GCs on the hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis is considered to be mediated through two different intracellular receptors: the mineralocorticoid receptor (MR) and GC receptor (GR) [2]. GR, a family member of conserved nuclear receptors, is a nuclear transcription factor widely present in various somatic cells [3] and can only be activated under high concentration GC stimulation [4]. GRα and GRβ are two subtypes of GC. GRα is more abundant than GRβ, and it can bind to GC and activate the response of related genes [5,6]
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