Glomalin-related soil protein influences the accumulation of polycyclic aromatic hydrocarbons by plant roots

Abstract

Studies have demonstrated that the inoculation of soil with arbuscular mycorrhizal fungi (AMF) enhances the content of glomalin-related soil protein (GRSP), which in turn elevates the availability of polycyclic aromatic hydrocarbons (PAHs) in soil. However, few studies have examined the influence of GRSP on PAH accumulation by plants and their tissues. Understanding of this issue would provide new perspectives on the role of GRSP in PAH uptake by plants at contaminated sites. This investigation was the first observational study of the GRSP-influenced PAH accumulation in roots of ryegrass (Lolium multiflorum Lam.). GRSP (0–120 mg/L) enhanced the root PAH accumulation in a GRSP-concentration-dependent manner, based on the observed root concentrations and root concentration factors (RCFs). The greatest enhancement of ΣPAH accumulation appeared at 40 mg/L of the total GRSP (T-GRSP) and 80 mg/L of the easily extracted GRSP (EE-GRSP), respectively. The weakly and strongly adsorbed fractions accounted for 88.8–94.4%, while the absorbed fraction contributed no >11.2% of total PAH accumulation in roots. The capacity of PAH adsorption on roots was enlarged in the presence of GRSP (0–120 mg/L). As the adsorbed fraction dominated the total PAH contents in roots overwhelmingly, the GRSP-induced changes in root PAH accumulation were ascribed to GRSP-affected PAH sorption by roots.