Abstract
A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1.
Highlights
A great number of pharmacological compounds is found in the Brazilian marine diversity
A biodiversidade marinha brasileira abrange uma linha de costa de aproximadamente 9.198 km, a qual se encontra banhada por águas quentes e frias das bordas tropicais e subtropicais do Atlântico Sul Ocidental
Os GAGs são constituintes naturais da matriz extracelular em todos os organismos que apresentam organização tecidual (DIETRICH; DIETRICH, 1976; MEDEIROS et al, 2000; TINGHO et al, 2005), sendo detectados no líquido sinovial, cartilagens e pele
Summary
Dois exemplares adultos de palombeta (peso = 142,00 g; comprimento = 25,75 cm) e um de guaiúba. (peso = 327,00 g; comprimento = 39,70 cm) foram adquiridos de estabelecimentos comerciais de Fortaleza, Estado do Ceará. A pesquisa foi realizada no Laboratório de Carboidratos e Lectinas (CarboLec) do Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará. A remoção e tratamento da pele do músculo dos peixes foram realizadas segundo Rodrigues et al (2009b e 2011). A pele foi armazenada em frasco fechado para posterior extração dos GAGs
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