GLABROUS1 from Rosa roxburghii Tratt regulates trichome formation by interacting with the GL3/EGL3 protein

Abstract

GLABROUS1 is an R2R3 MYB homolog that is essential for the initiation of trichome development. In this study, we used RACE to clone a full-length cDNA from Rosa roxburghii Tratt, termed RrGL1, which was 1013 bp, including an open reading frame of 792 bp that encoded 263 amino acids. In situ hybridization, corresponding with qRT-PCR results, revealed that RrGL1 transcripts were mainly expressed in petiole, leaf, and stem. RrGL1 expression levels at various fruit developmental stages in R. roxburghii were also evaluated. RrGL1 was highly expressed in young fruit, and the expression level decreased with fruit maturation. The overexpression of RrGL1 was able to functionally complemented the Arabidopsis thaliana gl1−/− mutant in trichome formation. RrGL1 was located in the cell nucleus with analysis of subcellular localization and physically interacted with A. thaliana GL3/EGL3 in the yeast two-hybrid assay, implying that RrGL1 might exert functions by forming a MYB-basic helix-loop-helix complex in trichome initiation. The formation of prickles in R. roxburghii is similar to that of Arabidopsis trichome.These results provided a foundation for further research on the molecular mechanisms underlying the formation and development of prickles that could assist and cultivate in the genetic engineering of prickle-free fruits.