Abstract
Abstract The greatest diversity of ferns occurs in the tropics, where approximately three in four of the species are found. In the Brazilian flora, the ferns have megadiversity, being an important plant group. The objective was to evaluate the spores germination of ferns Polypodium aureum in different types of culture medium. The sporangia were removed from fertile fronds and sterilized with sodium hypochlorite 0.57% during 30 minutes. After sterilization, the sporangia were seeded in culture media agar, MS, SH, White and B5. The experimental design was completely randomized being composed of 5 treatments (agar media, B5, SH, White and MS) and 10 replications. The ratings occurred every 3 days over a period of 45 days, counting the number of spores germinated. After this period, the germination percentage, germination time on medium, germination speed index and timing of germination were calculated. The germination percentage and the index of synchronization have not changed in function of different culture media. The medium White presented the highest average for the germination speed index whereas the agar media, MS, SH and B5 had the same germination speeds. The percentage of mortality was higher in MS medium, the White medium presented the lowest percentage of mortality, and the media composed by agar, SH and B5 showed intermediate values. The lowest average germination time occurred at the media SH and B5, since the environment composed by agar showed higher average germination time. The media MS and White showed similar results to the agar, SH and B5. Based on the obtained results, it can be concluded that the White medium is the most effective on the germination of spores of Polypodium aureum.
Highlights
The Amazonian ferns, Polypodium aureum Lowe, are native from Brazil, from Polypodiaceae family, are perennial, vigorous herbaceous vegetations, and with rhizomes
The ferns of the species P. aureum can reproduce in two ways, the first way is by cuttings and the second way is by germination of spores
The Sporangia were removed from the fronds using a brush with stiff bristles, placed approximately 30 mg of Sporangia within a 3 mL syringe to facilitate the disinfection of the sporangia, the sporangia were sterilized in a solution of sodium hypochlorite 0.57% during 30 min, which were obtained from a commercial solution diluted to 20%
Summary
The Amazonian ferns, Polypodium aureum Lowe, are native from Brazil, from Polypodiaceae family, are perennial, vigorous herbaceous vegetations, and with rhizomes It has large rhizomes and follicles, with large and crooked fronds (compound leaves). These species of ferns can be grown in pots containing good soil fertility and enriched with organic matter, are a little colder tolerant (Lorenzi et al, 2008). The cutting is a technique for vegetative propagation, which consists of stem and root segments planting of the plant matrix, giving origin to a new plant, from the rooting of the same This method is widely used in the creations of seedlings of plants, especially trees and ornamental plants (Hartmann et al, 2002)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
