Abstract
The respective roles of germinal vesicle (GV) nucleoplasm dispersion and intracellular alkalinization in the acquisition of cytoplasmic maturity by oocytes of the prosobranch mollusk Patella vulgata have been investigated in experiments involving premature fertilization of prophase-blocked oocytes. These were then either enucleated and treated with 10 m M NH 4Cl, pH 8.5, or induced to break their germinal vesicle in the absence of any evoked intracellular pH change. Results indicate that male pronuclear decondensation, sperm aster differentiation and cleavage require both GV nucleoplasm dispersion and intracellular pH alkalinization. These data are discussed in relation to the respective roles of calcium, pH, and nucleoplasm during maturation and activation of invertebrate and vertebrate oocytes.
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