Abstract
To develop polysaccharide-based membranes that allow controlled and localized delivery of gentamicin for the treatment of post-operative bone infections. Membranes made of gellan gum (GUM), sodium alginate (ALG), GUM and ALG crosslinked with calcium ions (GUM+Ca and ALG+Ca, respectively) as well as reference collagen (COL) were produced by freeze-drying. Mechanical properties, drug release, antimicrobial activity and cytocompatibility of the membranes were assessed. The most appropriate handling and mechanical properties (Young's modulus, E=92±4MPa and breaking force, F MAX =2.6±0.1N) had GUM+Ca membrane. In contrast, COL membrane showed F MAX =0.14±0.02N, E=1.0±0.3MPa and was deemed to be unsuitable for antibiotic delivery. The pharmacokinetic data demonstrated a uniform and sustainable delivery of gentamicin from GUM+Ca (44.4±1.3% within 3weeks), while for COL, ALG and ALG+Ca membranes the most of the drug was released within 24h (55.3±1.9%, 52.5±1.5% and 37.5±1.8%, respectively). Antimicrobial activity against S. aureus and S. epidermidis was confirmed for all the membranes. GUM+Ca and COL membranes supported osteoblasts growth, whereas on ALG and ALG+Ca membranes cell growth was reduced. GUM+Ca membrane holds promise for effective treatment of bone infections thanks to favorable pharmacokinetics, bactericidal activity, cytocompatibility and good mechanical properties.
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