Genotyping detection and phylogenetic analysis of human Bocavirus among inpatient children with severe acute respiratory infection

Abstract

Objective To investigate the prevalence,epidemiological and phylogenetic characteristics of human Bocavirus (HBoV) among inpatient children with severe acute respiratory infection (SARI).Methods Two hundred and fifty-nine nasopharyngeal aspirates (NPAs) were collected from inpatient children with SARI in Beijing Children' s Hospital.Human Bocavirus was genotypic detected by Nested PCR and sequenced followed by the epidemiological and phylogenetic analysis.Results HBoV was detected in 56 of 259 NPAs from inpatient children with SARI.The positive rate was 21.6％,95％ CI (16.0％-27.3％).Most of the HBoV infection children were younger than two years.The co-infection rate of HBoV with other respiratory viral pathogens was 94.6％.And 96.4％ (54/56) were positive for HBoV1.Only one of HBoV2 and one of HBoV3 positive case was found.The sequence evolution of partial VP1/VP2 fragment was relative conserved by phylogenetic analysis,Conclusion HBoV was common detected viral pathogens among children with SARI.HBoV1 was more prevalent than other genotype.However,more investigation was needed to determine the etiological role of HBoV infection among inpatient children with SARI. Key words: Bocavirus,human; Respiratory tract infections; Genotype; Phylogeny