Abstract
Cronobacter sakazakii, an opportunistic foodborne pathogen and a main cause of meningitis in neonates, is usually isolated from powdered milk infant formula (PMIF). At the present study, C. sakazakii were isolated from imported and domestically produced PMIF samples and identified by detection of ompA gene using real‐time PCR SYBR green melting curve following the evaluation of antimicrobial susceptibility and genotyping of the isolates employing BOX‐PCR and RAPD methods. We detected totally 5% contamination rate and a significantly higher prevalence of C. sakazakii in bulky imported domestically packaged PMIF samples. Also, our isolates were recognized as multidrug‐resistant pathogen completely resistant to ampicillin and amoxicillin; and intermediately resistant to ciprofloxacin and tetracycline antimicrobials. Genotype clustering patterns of bulky imported and imported product isolates were identical by both genotyping methods. Far genetic relatedness of domestic isolate to other isolates and the reference strain indicated higher genetic diversity of the domestic isolate genome. Multidrug resistance and diverse population genetic make complicated situation for determination of strategies for infectious disease prevention.
Highlights
Not appreciated by pediatric and nutrition specialists, powdered milk infant formula (PMIF) is the most recommended alternative of breast milk for neonatal feeding (Lönnerdal, 2016)
Genotypic and antimicrobial resistance characterizations of Cronobacter sakazakii isolated from powdered milk infant formula: A comparison between domestic and imported products
C. sakazakii were isolated from imported and domestically produced PMIF samples and identified by detection of ompA gene using real-time PCR SYBR green melting curve following the evaluation of antimicrobial susceptibility and genotyping of the isolates employing BOX-PCR and RAPD methods
Summary
Not appreciated by pediatric and nutrition specialists, powdered milk infant formula (PMIF) is the most recommended alternative of breast milk for neonatal feeding (Lönnerdal, 2016). Genotypic and antimicrobial resistance characterizations of Cronobacter sakazakii isolated from powdered milk infant formula: A comparison between domestic and imported products Babak Pakbin1 | Razzagh Mahmoudi2 | Shaghayegh Mousavi3 | Samaneh Allahyari4 | Zahra Amani1 | Amir Peymani2 | Peyman Qajarbeygi5 | Zahra Hoseinabadi4
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