Abstract
Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of one 2,990,570-bp chromosome and four plasmids. The comparative genomic and phylogenetic analysis revealed that L. brevis CD0817 was not very conserved with low GABA-producing L. brevis strains. A significant divergence was that CD0817 harbors only the gadCA operon whereas the low GABA-producing L. brevis strains contain the operon and gadB. The gadB seemed to only marginally contribute to the accumulation of GABA. The high GABA production ability of CD0817 may be associated with its extraordinary genome.
Highlights
Gamma-aminobutyric acid (GABA), a four-carbon nonprotein amino acid diffusely distributed in nature, is the major inhibitory neurotransmitter in the mammalian central nervous system (Li and Cao 2010)
Over the past three decades, the bio-manufacture of GABA by using lactic acid bacteria (LAB) has been vigorously pursued due to the fact that LAB are generally regarded as safe (Li and Cao 2010)
Glutamic acid decarboxylase (GAD) system comprising glutamate/GABA antiporter and GAD enzyme is responsible for the synthesis of GABA in microbial cells: glutamate is transported into a cell through the antiporter, subsequently decarboxylation occurs, the decarboxylated product is exported from the cell by the antiporter (Small and Waterman 1998)
Summary
Gamma-aminobutyric acid (GABA), a four-carbon nonprotein amino acid diffusely distributed in nature, is the major inhibitory neurotransmitter in the mammalian central nervous system (Li and Cao 2010). Over the past three decades, the bio-manufacture of GABA by using lactic acid bacteria (LAB) has been vigorously pursued due to the fact that LAB are generally regarded as safe (Li and Cao 2010). Numerous LAB strains, notably lactobacilli belonging to L. brevis (Wu et al 2015), L. plantarum (Siragusa et al 2007), L. paracasei (Komatsuzaki et al 2005) and L. buchneri (Zhao et al 2015) have been applied to biosynthesize GABA. GadCA form an operon while gadB is separate and far from the operon circa 1.7 Mb in a L. brevis genome (Li et al 2013)
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