Abstract
Advances in next-generation sequencing techniques have allowed for the generation of genome-wide sequence data, to gain insight into the dynamics influencing genetic structure and the local adaptation of marine fish. Here, using genotyping-by-sequencing (GBS) technique, we identified 31,119 single nucleotide polymorphisms (SNPs) for Sebastiscus marmoratus in 59 individuals from three populations in Chinese coastal waters. Based on all SNPs, there was little evidence of genetic differentiation among populations. However, outlier tests revealed 329 SNPs putatively under divergent selection across populations. Structural and phylogenetic topology analyses based on the outliers showed clear genetic differentiation among populations. Gene Ontology (GO) annotation results revealed that most of these outliers are known or hypothesized to be involved in metabolic process. Together with previous work using mitochondrial cytochrome b sequences, the present results further suggest that the population structure is strongly influenced by locally adaptive pressure. Overall, adaptive evolution in a heterogeneous environment plays an important role in inducing genetic differentiation among local populations. This study increases understanding of the factors (including gene flow and local adaptation) promoting and constraining population genetic differentiation in marine organisms.
Highlights
Inferring the degree of genetic differentiation among populations of marine fish species is key to successfully managing fishery resources, allowing the identification of management units, assignment of individuals to geographic regions, and detection of product mislabeling and fraud[1,2,3]
Most previous population genetic studies of S. marmoratus were based on a handful of microsatellites, mtDNA loci and amplified fragment length polymorphism (AFLP) markers, which obtained insufficient and inconsistent results[31, 32]
We identified a total of 31,119 single nucleotide polymorphisms (SNPs) for population genomic analyses, of which 329 outlier SNPs were identified as candidates for selection
Summary
Inferring the degree of genetic differentiation among populations of marine fish species is key to successfully managing fishery resources, allowing the identification of management units, assignment of individuals to geographic regions, and detection of product mislabeling and fraud[1,2,3]. In this study, we sampled a total of 59 S. marmoratus individuals from three populations from the northernmost to the southernmost populations across its distribution range in China and performed genotyping by sequencing (GBS) to examine genome-wide population structure and test for genetic signatures of local adaptation.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
