Abstract

The DNAs of five single-nucleocapsid baculoviruses (S-NPVs) from plusiine hosts were compared. Restriction endonuclease fragments of three geographical isolates of Trichoplusia ni S-NPVs and isolates of Pseudoplusia incluens and Syngrapha selects viruses were compared using a battery of enzymes which included EcoRI, BamHI, SalI, SmaI, and HindIII. The results show that T. ni and P. includens S-NPVs are distinct, but related, viruses and that the S. selecta virus is a strain of T. ni S-NPV. Minor differences in geographical isolates of T. ni S-NPVs were detected. The data are generally consistent with the host-range properties of these viruses, suggesting that the combination of DNA restriction profiles and host-range matrices may provide a valuable tool for the identification and cataloging of the plusiine S-NPVs.

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