Abstract
Circoviruses are highly prevalent porcine and avian pathogens. In recent years, novel circular ssDNA genomes have recently been detected in a variety of fecal and environmental samples using deep sequencing approaches. In this study the identification of genomes of novel circoviruses and cycloviruses in feces of insectivorous bats is reported. Pan-reactive primers were used targeting the conserved rep region of circoviruses and cycloviruses to screen DNA bat fecal samples. Using this approach, partial rep sequences were detected which formed five phylogenetic groups distributed among the Circovirus and the recently proposed Cyclovirus genera of the Circoviridae. Further analysis using inverse PCR and Sanger sequencing led to the characterization of four new putative members of the family Circoviridae with genome size ranging from 1,608 to 1,790 nt, two inversely arranged ORFs, and canonical nonamer sequences atop a stem loop.
Highlights
IntroductionThe virions consist of naked nucleocapsids of about 20 nm in diameter, with a circular single stranded DNA (ssDNA) genome of approximately 2.0 kb [1]
Viruses of the Circoviridae family are known to infect a wide range of vertebrates
As shown in the tree, it was observed the arrangement of five main groups with clusters II (4 sequences), VI (3 sequences) and VII (2 sequences) falling into the clade of cycloviruses, in contrast to clusters I (13 sequences) and V (2 sequences) that formed distinct and distant groups from those formed by circoviruses and cycloviruses
Summary
The virions consist of naked nucleocapsids of about 20 nm in diameter, with a circular single stranded DNA (ssDNA) genome of approximately 2.0 kb [1]. They have an ambisense genome organization containing two major open reading frames (ORFs) inversely arranged, responsible for encoding the replicase (Rep) and capsid (Cap) proteins, and are separated by a 3’ intergenic region (IGR) between the stop codons and a 5’ IGR between the start codons [2]. Avian circoviruses, within the genus Gyrovirus, have been
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