Abstract
BackgroundIn recent years, the molecular mechanism of plant growth and development has been reported in detail. GRAS genes, a plant-specific family of transcription factor, play critical roles in the process. GRAS transcription factors are associated with axillary shoot meristem formation, radial root patterning, phytohormones (gibberellins) signal transduction, light signaling, and abiotic or biotic stress. ObjectiveHere, we firstly investigated GRAS gene family in Dendrobium catenatum, an important medicinal and flowering orchid in China. MethodsThe GRAS gene family in D. catenatum was cloned based on RNA-Seq data. Selected GRAS genes were introduced into Escherichia coli to express proteins. ResultsBased on phylogenetic relationship with the Arabidopsis and Oryza GRAS family members, 47 GRAS genes from D. catenatum are identified and their deduced proteins are classified into 11 subgroups. Most of these GRAS genes contain one exon and closely related members in the phylogenetic tree have similar motif composition. Our result also reveals that GRAS genes in D. catenatum are widely distributed and expressed in different tissue. In addition, 35 GRAS genes are successfully cloned from different subgroups and 7 DoGRAS fusion proteins are induced using E. coli system. Moreover, 8 genes were up-regulated in different tissue following exposure to heat and salt stresses. ConclusionOur findings provide valuable information and candidate genes for future functional analysis for improving the resistance of D. catenatum growth.
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