Abstract
Phosphatidylinositol-specific phospholipase C (PI-PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate. It plays an important role in plant development and abiotic stress responses. However, systematic analysis and expression profiling of the phospholipase C (PLC) gene family in soybean have not been reported. In this study, 12 putative PLC genes were identified in the soybean genome. Soybean PLCs were found on chromosomes 2, 11, 14 and 18 and encoded 58.8–70.06 kD proteins. Expression pattern analysis by RT-PCR demonstrated that expression of the GmPLCs was induced by PEG, NaCl and saline-alkali treatments in roots and leaves. GmPLC transcripts accumulated specifically in roots after ABA treatment. Furthermore, GmPLC transcripts were analyzed in various tissues. The results showed that GmPLC7 was highly expressed in most tissues, whereas GmPLC12 was expressed in early pods specifically. In addition, subcellular localization analysis was carried out and confirmed that GmPLC10 was localized in the plasma membrane in Nicotiana benthamiana. Our genomic analysis of the soybean PLC family provides an insight into the regulation of abiotic stress responses and development. It also provides a solid foundation for the functional characterization of the soybean PLC gene family.
Highlights
The PLC gene family is a subfamily of the phospholipase superfamily, which includes phospholipase D, phospholipase C, phospholipase A1 and phospholipase A2
A similar phenomenon was found in Arabidopsis; AtPLC1, AtPLC4 and AtPLC5 are located in a 12 kb fragment on chromosome 5 [33]
Conversed domain and phylogenetic analyses were carried out and verified that all soybean PLC genes belong to the phosphatidylinositol-specific phospholipase C (PI-PLC) subfamily
Summary
The PLC (phospholipase C) gene family is a subfamily of the phospholipase superfamily, which includes phospholipase D, phospholipase C, phospholipase A1 and phospholipase A2. PLCs are divided into phosphatidylinositol-specific phospholipase C (PI-PLC) and non-specific phospholipase (NPC) by different hydrolysis sites [1]. NPC hydrolyzes the membrane lipids phosphatidylcholine and phosphatidylethanolamine in a calcium-independent manner [1]. It plays an important role in the supply of inorganic phosphate in the root plasma membrane during phosphate deprivation [2]. PI-PLC, named PLC, exists in both animals and plants. PLOS ONE | DOI:10.1371/journal.pone.0138467 September 30, 2015
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