Abstract
Our current study is to identify clinical significance of minichromosome maintenance (MCM) gene expression in Lung Adenocarcinoma (LUAD) using genome-wide RNA sequencing (RNA-seq) dataset and bioinformatics analysis tools. The biological function and potential process for function of the MCM1-10 were identified by multiple bioinformatics analysis tools. Clinical significance and molecular mechanism of the MCM1-10 were investigated by the RNA-seq dataset of LUAD from The Cancer Genome Atlas. Functional assessment substantiated involvement of MCM1-10 in cell cycle progression and DNA replication, and co-expressed with each other. We also observed that the MCM1-10 were dysregulation in LUAD tumor tissues, and may be have diagnostic implications in LUAD. Prognosis analysis in TCGA and KM plotter cohorts suggest that high abundance of MCM5, MCM8 and MCM4 notably correlated to poor LUAD overall survival. Mechanistic exploration of MCM4, MCM5, and MCM8 by gene set enrichment analysis suggests that these genes may influence the LUAD prognosis by regulating the cell cycle, DNA replication and other multiple biological processes and pathways. In comclusion, our study suggests that MCM1-10 can serve as diagnostic biomarkers for LUAD patients. Of them, MCM4, MCM5, and MCM8 may act as potential prognostic indicators for LUAD.
Highlights
Worldwide, Lung cancer is known to have the highest rate of morbidity and mortality worldwide
Biological processes investigation by gene ontology (GO) term enrichment analysis using DAVID v6.8 suggested that these minichromosome maintenance (MCM) genes were significantly associated with cell cycle progression through G1/S, DNA damage response and DNA replication related biological events namely DNA unwinding involved in DNA replication, replication origin binding, initiation of replication, helicase activity, and DNA duplex unwinding (Fig 1A)
It is composed of six subunits, including MCM2, MCM3, MCM4 (CDC21), MCM5 (CDC46), MCM6 (Mis5) and MCM7 (CDC47), and exhibit the helicase activity of replicating DNA.[26]MCM8, MCM9, MCM1 and MCM10 are found in multicellular organisms and play crucial functions in DNA replication.[7,27]Our bioinformatics analysis demonstrated that MCM1-10 is involved in cell cycle and replication of DNA
Summary
Lung cancer is known to have the highest rate of morbidity and mortality worldwide.
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