Abstract
Members of the lectin receptor-like kinase (LecRLKs) family play a vital role in innate plant immunity. Few members of the LecRLKs family have been characterized in rice and Arabidopsis, respectively. However, little literature is available about LecRLKs and their role against fungal infection in cucumber. In this study, 60 putative cucumber LecRLK (CsLecRLK) proteins were identified using genome-wide analysis and further characterized into L-type LecRLKs (24) and G-type LecRLKs (36) based on domain composition and phylogenetic analysis. These proteins were allocated to seven cucumber chromosomes and found to be involved in the expansion of the CsLecRLK gene family. Subcellular localization of CsaLecRLK9 and CsaLecRLK12 showed green fluorescence signals in the plasma membrane of leaves. The transcriptional profiling of CsLecRLK genes showed that L-type LecRLKs exhibited functional redundancy as compared to G-type LecRLKs. The qRT-PCR results indicated that both L- and G-type LecRLKs showed significant response against plant growth-promoting fungi (PGPF-Trichoderma harzianum Rifai), powdery mildew pathogen (PPM—Golovinomyces orontii (Castagne) V.P. Heluta), and combined (PGPF+PPM) treatments. The findings of this study contribute to a better understanding of the role of cucumber CsLecRLK genes in response to PGPF, PPM, and PGPF+PPM treatments and lay the basis for the characterization of this important functional gene family.
Highlights
Powdery mildew of cucumber (Cucumis sativus L.) caused by Golovinomyces orontii (Castagne) V.P
After mining the orthologous genes of Arabidopsis, 60 putative CsLecRLK protein sequences were identified in total, which were further classified into L-type lectin receptor-like kinase (LecRLKs) (n = 24)
Previous studies on genome-wide analysis have identified LecRLK genes in many crops, including Arabidopsis (75), wheat analysis have identified LecRLK genes in many crops, including Arabidopsis (75), wheat (263), (263), and rice (173) [4,6,21], which signifies that differences in copy numbers of LecRLKs might be due to variation in genome size and expansion rate
Summary
Powdery mildew of cucumber (Cucumis sativus L.) caused by Golovinomyces orontii (Castagne) V.P. Heluta. Is a devastating fungal disease across the world that causes major damage to most of the Cucurbitaceae plants, which affects the quality of the fruit, thereby resulting in high yield loss in both greenhouse and field crops [1]. The management of powdery mildew disease is a challenging task in the cucumber growing regions. Fungicide, flusilazole, and potassium salts as inducers are effectively recommend to overcome or reduce the disease problem. The application of such chemicals can result in severe negative impacts on human health and environmental contamination. Eco-friendly disease management practices are essential to overcome with this disease
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
