Abstract
Receptor like kinases (RLKs) are preserved upstream signaling molecules which regulate several biological processes from plant development to various stress adaptation programs. Non arginine aspartate (non-RD) a prominent class of RLKs plays a significant role in disease resistance and apoptosis in plants. In present investigation, a comprehensive in silico analysis for non-RD Kinase gene family as well as identification of gene structures, sequence similarity, chromosomal localization, gene duplication analysis, promoter analysis, transcript expression profiles and phylogenic studies were done. In this study, twenty-six genes were observed on nine out of twelve chromosomes. All these genes were clustered into five subfamilies under large monophyletic group termed as Interleukin-1 Receptor-Associated Kinase (IRAK) family. Some of the important physiochemical properties of twenty-six proteins are determined and ranged in the following order: (a) Amino acids size ranged from (620 to 1781) (b) Molecular weight ranged as of (70.11 to 197.11 KDa) and (c) Theoretical PI ranged from (5.69 to 8.63) respectively. Structural diversity in genomic structure among non-RD kinase gene family was identified and presence of pathogen induced cis regulatory elements including STRE, MYC, MYB, and W box were found. Expression profiles revealed the potential ability of three genes CaRLK1 from LRRXII and CaRLK15,16 from stress antifung subfamily were pointedly upregulated beyond the severe stress time period (9 DAI) in anthracnose resistant genotype PBC-80 in response to Colletotrichum truncatum infection. Subsequently, in silico studies from the available genome sequencing data helped us to identify candidate genes tangled in inducing disease resistance.
Highlights
One of the major challenges in the 21st century, to global food security and agricultural sustainability is to develop economically important high yielding varieties which are stable with broad-spectrum of resistance
From PDB database optimized length of known receptor like kinase sequences ranged from 100-1000 amino acids [S10]
Most of the candidate Receptor Like kinases (RLKs) genes involved in primary immune responses associated with disease resistance belong to non-RDclass kinases
Summary
One of the major challenges in the 21st century, to global food security and agricultural sustainability is to develop economically important high yielding varieties which are stable with broad-spectrum of resistance. Hot pepper is a commercially important vegetable crop grown worldwide for its indispensable nutritional and therapeutic values, but year by year its production has been reduced due to several biotic stresses [1]. As a part of its development of anthracnose resistant hot pepper remained as one of the major tasks to be resolved by modern agriculture practices prevailing worldwide. Knowledge of understanding the defensive signaling mechanisms employed by resistant plants while encountering the attacking pathogen by advanced molecular and computational techniques paved a ray of hope to address these challenges [3].
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