Abstract
Brassinazole-resistant (BZR) family genes encode plant-specific transcription factors (TFs), play essential roles in the regulation of plant growth and development, and have multiple stress-resistance functions. Nicotiana benthamiana is a model plant widely used in basic research. However, members of the BZR family in N. benthamiana have not been identified, and little is known about their function in abiotic stress. In this study, a total of 14 BZR members were identified in the N. benthamiana genome, which could be divided into four groups according to a phylogenetic tree. NbBZRs have similar exon-intron structures and conserved motifs, and may be regulated by cis-acting elements such as STRE, TCA, and ARE, etc. Organ-specific expression analysis showed that NbBZR members have different and diverse expression patterns in different tissues, and most of the members are expressed in roots, stems, and leaves. The analysis of the expression patterns in response to different abiotic stresses showed that all the tested NbBZR members showed a significant down-regulation after drought treatment. Many NbBZR genes also responded in various ways to cold, heat and salt stress treatments. The results imply that NbBZRs have multiple functions related to stress resistance.
Highlights
The plant steroid hormones, Brassinosteroids (BRs), play an important role in regulating plant growth and development, and biotic and abiotic stress responses [1]
The fully activated Brassinosteroid insensitive 1 (BRI1)/BAK1 complex triggers a series of downstream phosphorylation events, which lead to the regulation of a large number of genes [2,6,7,8]
The BZR transcription factor contains a BES1_N superfamily domain, and we identified possible BZR members in N. benthamiana based on this standard
Summary
The plant steroid hormones, Brassinosteroids (BRs), play an important role in regulating plant growth and development, and biotic and abiotic stress responses [1]. The BR receptor Brassinosteroid insensitive 1 (BRI1) is a leucine-rich, repeated, receptor-like kinase (LRR RLK) located in the plasma membrane. When BR molecules are detected, it recruits another LRR RLK BRI1- associated receptor kinase (BAK1) to form a receptor complex. Brassinazole resistant 1 (BZR1) and Brassinosteroid insensitive 1-ethyl methanesulfonate-suppressor 1 (BES1) are two downstream transcription factors (TFs) belonging to the Brassinazoleresistant (BZR) TF family. They can be dephosphorylated by protein phosphatase when BR is detected [9]. Dephosphorylated BZR1 and BES1 accumulate in the nucleus and directly bind to cis-elements to regulate plant growth and development [10]
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