Abstract
The AINTEGUMENTA-like (AIL) proteins, which belong to the AP2 family, play important roles in regulating the growth and development of plant organs. The AIL family has not yet been comprehensively studied in rapeseed (Brassica napus), an allotetraploid and model organism for the study of polyploid evolution. In the present study, 99 AIL family genes were identified and characterized from B. rapa, B. oleracea, B. napus, B. juncea, and B. nigra using a comprehensive genome-wide study, including analyses of phylogeny, gene structure, chromosomal localization, and expression pattern. Using a phylogenetic analysis, the AIL genes were divided into eight groups, which were closely related to the eight AtAIL genes, and which shared highly conserved structural features within the same subfamily. The non-synonymous/synonymous substitution ratios of the paralogs and orthologs were less than 1, suggesting that the AIL genes mainly experienced purifying selection during evolution. In addition, the RNA sequencing data and qRT-PCR analysis revealed that the B. napus AIL genes exhibited organ- and developmental stage-specific expression patterns. Certain genes were highly expressed in the developing seeds (BnaAIL1, BnaAIL2, BnaAIL5, and BnaAIL6), the roots (BnaANT, BnaAIL5, and BnaAIL6), and the stem (BnaAIL7B). Our results provide valuable information for further functional analysis of the AIL family in B. napus and related Brassica species.
Highlights
Plant growth and developmental processes are influenced by the complex external environment and internal developmental factors [1, 2]
The amino acid sequences of the AIL proteins were obtained from The Arabidopsis Information Resource (TAIR10) database and used as queries for a BLASTp search of the whole Brassica genome sequences stored in the Brassica database [34], including the B. rapa genome V3.0, B. oleracea genome V1.1, B. napus genome V5, B. juncea genome V1.5, and B. nigra genome V1.1, respectively
Eight A. thaliana AIL family protein sequences were acquired from the TAIR10 database and used as queries to identify the AIL family in various Brassica species (B. rapa, B. oleracea, B. napus, B. juncea, and B. nigra) using the BLASTp program
Summary
Plant growth and developmental processes are influenced by the complex external environment and internal developmental factors [1, 2]. Deciphering the molecular networks contributing to plant growth and development is an important research goal. Genome-wide analysis and expression profiles of AIL genes in Rapeseed. Universities of the Central South University (XDJK2020B030), Chongqing Postdoctoral Research Foundation (CN) (XmT2018057), 111 project (CN) (B12006), and Modern Agro-industry Technology Research System (CN) (CARS-12). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
