Abstract
BackgroundB. napus (oilseed) is an important source of edible vegetable oil, and its nutritional and economic value is determined by its fatty acid composition and content.ResultsUsing the Brassica 60 K SNP array, we performed a genome-wide association study of fatty acid composition in a population of 520 genetically diverse oilseed accessions. Using the PCA + K model in TASSEL 5.2.1, we identified 62 genomic regions that were significantly associated with the composition of seven fatty acids, and five consensus regions that mapped to the A2, A8, A9, C1, and C3 chromosomes, respectively, of the Brassica napus Darmor-bzh genome. We then identified 24 orthologs of the functional candidate genes involved in fatty acid biosynthesis, excluding BnaA.FAE1 and BnaC.FAE1 on the A8 and C3 homologous genome blocks, which are known to have critical roles in the fatty acid biosynthesis pathway, and potential orthologs of these genes (e.g., LACS9, KCR1, FAB1, LPAT4, KCS17, CER4, TT16, and ACBP5).ConclusionsOur results demonstrate the power of association mapping in identifying genes of interest in B. napus and provide insight into the genetic basis of fatty acid biosynthesis in B. napus. Furthermore, our findings may facilitate marker-based breeding efforts aimed at improving fatty acid composition and quality in B. napus.
Highlights
B. napus is an important source of edible vegetable oil, and its nutritional and economic value is determined by its fatty acid composition and content
Quantitative trait locus (QTL) mapping has yielded a vast amount of information on complex traits of rapeseed, such as oil content [7,8,9,10,11,12,13] and fatty acid composition [2,3,4, 14, 15], and the underlying quantitative trait locus (QTL) were mapped to all 19 linkage groups of B. napus
The results showed that significant Single Nucleotide Polymorphism (SNP) on chromosome A8 were located in a haplotype block of 437 kb, from 10.1 Mb to 10.5 Mb, and covered three key genes, ketoacyl-CoA synthase 18 (KCS18) (FAE1, BnaA08g11130D), ketoacyl-CoA synthase 17 (KCS17) (BnaA08g11140D), and CER4 (BnaA08g11440D)
Summary
B. napus (oilseed) is an important source of edible vegetable oil, and its nutritional and economic value is determined by its fatty acid composition and content. The fatty acid composition of Brassica oil determines its physical, chemical, and nutritional qualities [2, 3]. Quantitative trait locus (QTL) mapping has yielded a vast amount of information on complex traits of rapeseed, such as oil content [7,8,9,10,11,12,13] and fatty acid composition [2,3,4, 14, 15], and the underlying QTLs were mapped to all 19 linkage groups of B. napus. Few of the detected QTLs have been successfully used in rapeseed breeding programs [10]
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