Abstract
Superoxide dismutase (SOD) is an important enzyme that acts as the first line of protection in the plant antioxidant defense system, involved in eliminating reactive oxygen species (ROS) under harsh environmental conditions. Nevertheless, the SOD gene family was yet to be reported in rapeseed (Brassica napus L.). Thus, a genome-wide investigation was carried out to identify the rapeseed SOD genes. The present study recognized 31 BnSOD genes in the rapeseed genome, including 14 BnCSDs, 11 BnFSDs, and six BnMSDs. Phylogenetic analysis revealed that SOD genes from rapeseed and other closely related plant species were clustered into three groups based on the binding domain with high bootstrap values. The systemic analysis exposed that BnSODs experienced segmental duplications. Gene structure and motif analysis specified that most of the BnSOD genes displayed a relatively well-maintained exon–intron and motif configuration within the same group. Moreover, we identified five hormones and four stress- and several light-responsive cis-elements in the promoters of BnSODs. Thirty putative bna-miRNAs from seven families were also predicted, targeting 13 BnSODs. Gene ontology annotation outcomes confirm the BnSODs role under different stress stimuli, cellular oxidant detoxification processes, metal ion binding activities, SOD activity, and different cellular components. Twelve BnSOD genes exhibited higher expression profiles in numerous developmental tissues, i.e., root, leaf, stem, and silique. The qRT-PCR based expression profiling showed that eight genes (BnCSD1, BnCSD3, BnCSD14, BnFSD4, BnFSD5, BnFSD6, BnMSD2, and BnMSD10) were significantly up-regulated under different hormones (ABA, GA, IAA, and KT) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. The predicted 3D structures discovered comparable conserved BnSOD protein structures. In short, our findings deliver a foundation for additional functional investigations on the BnSOD genes in rapeseed breeding programs.
Highlights
IntroductionSeveral environmental cues, including abiotic and biotic traumas, are considered key influences affecting the plants’ productivity [1,2]
The current study identified 31 BnSOD genes in the rapeseed genome using eight
Out of 31 BnSODs, genes were located on the A subgenome, and genes were located on the C subgenome (Table 1)
Summary
Several environmental cues, including abiotic and biotic traumas, are considered key influences affecting the plants’ productivity [1,2]. The plant amends its homeostatic apparatus by developing an increased reactive oxygen species (ROS) in plant cells. ROS overproduction results in several molecular and cellular damages, and programmed cell death [3,4]. ROS such as superoxide anion, hydrogen peroxide, hydroxyl radical, perhydroxyl radicals, alkoxy radicals, peroxy radicals, singlet oxygen, and organic hydroperoxide, are considered to be major signaling molecules, Antioxidants 2021, 10, 1182.
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