Abstract
ABSTRACTA bovine rhinitis B virus, identified in a calf from Sweden, was genetically characterized. The complete polyprotein was recovered, and phylogenetic analysis showed that this virus has the highest similarity to a bovine rhinitis B virus previously identified in Mexico.
Highlights
A bovine rhinitis B virus, identified in a calf from Sweden, was genetically characterized
A nasal swab was collected from a BcoV-infected calf in Sweden, and the sample was treated with RNase A and DNase prior to RNA extraction
The reads were mapped to BcoV, and the remaining unmapped reads were de novo assembled using CLC Genomics Workbench and subjected to blastx searches using Diamond [4]
Summary
A bovine rhinitis B virus, identified in a calf from Sweden, was genetically characterized. A nasal swab was collected from a BcoV-infected calf in Sweden, and the sample was treated with RNase A and DNase prior to RNA extraction. The two amplified samples were prepared for sequencing using the Illumina Nextera XT DNA library preparation kit, and they were sequenced on the MiSeq platform. Between six and nine million reads were obtained per sample.
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