Genetic Variation of Rabbit Fish (Siganus canaliculatus Park, 1797) In the waters of Bone Bay and Makassar Strait

Abstract

This research aims to study the genetic variation of Rabbit fish in the waters of Makassar Strait and Bone Bay with Random Amplified Polimorfhic DNA (RAPD) method. The Rabbit fish samples obtained from three population, Makassar Strait waters (Parepare and Takalar) and Bone Bay waters (Luwu Regency). 9 fish from each population preserved with TNES urea buffer solution. Extraction of genomic DNA with Phenol-chloroform method. Between seven primer screenings, four primer selected for RAPD analysis. Genetic variation analyzed using TFPGA software (tool for population genetic analysis). The genetic relationship presented in dendogram. The research shows that the genetic variation of the fish (Siganus canaliculatus) with RAPD analysis resulting the highest similarity indeks (0.8644) obtained between Parepare and Takalar waters population, and the lowest (0.7290) between Parepare and Luwu waters population. The closest genetic relationship obtained from Parepare and Takalar waters population 0,1458 while the farthest obtained from Luwu and Parepare waters population with 0.3161 score. According to the genetic relationship obtained two main groups that are Makassar Strait and Bone Bay population. The two population in the Makassar Strait are the same group and separated from Bone Bay population.