Abstract
Potato cyst-nematodes (PCN), Globodera rostochiensis and G. pallida, were studied for genetic variation by using isoelectric focusing and specific enzyme staining. The calculation of coefficient of similarity on 15 enzymes and construction of a dendrogram for a range of PCN populations revealed low levels of genetic variation among the G. rostochiensis populations probably due to a limited number of introductions, while in the case of G. pallida, the level of genetic variation was greater and fewer populations displayed identical banding patterns. Interpopulation variation in zymograms was detected in only four enzyme systems (malic enzyme, malate dehydrogenase, cholinesterase and hexokinase). The remaining 11 enzyme systems were monomorphic in relation to qualitative differences in banding patterns.
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