Genetic Variation in Glutenin Protein Composition of Aestivum and Durum Wheat Cultivars and Its Relationship with Dough Quality

Abstract

Genetic variability of high molecular weight glutenin subunits and low molecular weight glutenin subunits composition at the Glu-1 loci in Triticum aestivum L., and T. durum L., wheat was studied using sodium dodecyl sulfate polyacrylamide gel electrophoresis and polymerase chain reaction based markers. The end use quality of wheat is mainly influenced by the composition of glutenin protein. Aestivum cultivar GW-273 showed highest gluten index (94.4%) and sedimentation value (61 mL). GW-273 and GW-322 showed highest Glu-1 score of 10 out of 10, indicating superior dough quality for bread making. Results from glutenin protein separation using electrophoresis revealed that selected Indian wheat cultivars were abundant in high molecular weight glutenin subunits AxNull allele, which is responsible for poor quality. Gene specific polymerase chain reaction using high molecular weight glutenin subunits and low molecular weight glutenin subunits primers showed Dx5 and Dy10 in only two cultivars GW-273 and GW-322, which is responsible for good dough quality. Sequencing of high molecular weight glutenin subunits Dx5 gene fragment showed four cysteine at the N-terminal end. Cysteine residues are helpful in intermolecular disulfide bond formation among different glutenin and gliadins proteins leading to good elasticity of dough.