GENETIC VARIATION BETWEEN TWO ECOTYPES OF EGYPTIAN CLOVER BY ISSR TECHNIQUE

Abstract

The ISSR markers have been used in order to determine genetic variation and relationship between two clover ecotypes. Six primers for ISSR-PCR technique and succeeded reliable amplicons for different types of Egyptian clover ecotypes. The results revealed polymorphisms level re-vealed by ISSR primers. HB-10 ISSR-primer was better than of the rest ISSR primers in polymorphic 100%. The Fahl monocut ecotype had 29 present bands, 2 absent bands in 31 total bands; also Fahl have two unique bands. The multicut ecotype Gemmiza 1, Giza 6 and Serw1 were given 20 present, 11 absent; 21 present and 10 absent and 23 present and 11 absent respectively. The three unique bands were appeared in two ecotypes. Fahl was given two with HB-11 and HB-13; the Serw multicut cultivar had one unique bands with HB-08. Similarity indices among the four Egyptian clover cultivars based on ISSR analysis was estimated the highest value was appeared between Fahl and Gemmiza 1 as well as Giza 6 and Serw 1 followed by Fahl and Serw 1. The lowest similarity value was appeared between Gemmiza 1 and Serw 1 followed by Gemmiza 1 and Giza 6.