Abstract
The role of genetic diversity is crucial for future improvements to meet societal demand for food security under a climate change scenario. Morphological, biochemical and molecular analyses have been suggested for the evaluation of genetic diversity in common bean (Phaseolus vulgaris L.) from Egypt. Twelve cultivars were collected from different five geographical isolated locations. These cultivars were evaluated using 20 morphological traits (15 qualitative and 5quantitative). The two cultivars P9 and P14 which were collected from Quesna and New Valley, respectively exhibited partially different morphologically features from other cultivars. The electrophoretic pattern of seed storage proteins indicated 45 different molecular weight bands, ranging from 309.7 kDa to 44.3 kDa with the presence of 4 unique bands and 41 polymorphic bands. ISSR results revealed a high level of polymorphism (69.1%) using six ISSR primers, which produced a total of 117 bands; out of them 85 bands are polymorphic, 25 monomorphic and 7 unique. Cluster analysis using CAP software program based on combined data revealed two groups. The obtained data confirmed that morphological, SDS-protein and ISSR markers are important tools for genetic analysis for common bean and recommended to give accurate results.
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