Genetic Variants at Exon 3 in AAT Gene in Relation to Milk Production Traits in Sahiwal and Karan Fries Cattle

Abstract

Background: The present study pertained to records on milk production and milk constituents of 100 Sahiwal cattle and 115 Karan Fries cattle, the data collected over a period of 2004 to 2016 from Animal Genetics and Breeding division from ICAR-National Dairy Research Institute, Karnal Haryana. Methods: Good quality genomic DNA was used for amplification of exon 3 of AAT gene (474bp) by polymerase chain reaction under optimized conditions and Sequenced data analysis for SNPs detection. The analysis was carried out with appropriate Statistical method using software’s in the computer centre of the institute under the Restricted Maximum Likelihood Method (REML), Estimation of breeding value, Association Estimation, Effect of genotypes on Breeding Value. Result: In Sahiwal SNP at position G6997C was highly associated for FL305DMY, FLTMY, and FL305DSNFY. The mean ± SE of GG genotype for FL305DMY, FLTMY, FL305DFY, FL305DSNFY and FL305DPYwere found to be 1748.45±6.47, 1962.30±8.47, 100.33±0.44, 154.41±0.09 and 43.99±0.10 respectively and for GC genotype 1860.17±5.86, 2050.44±7.66, 100.35±0.39, 155.28±0.08 and 43.82±0.10 respectively. GG genotype was superior for FL305DPYand Heterozygous GC genotype was superior for rest of other traits. In Karan Fries cattle SNP at position G6997C was highly associated for FL305DMY, FLTMY, FL305DSNFY and FL305DPY. The mean ± SE of GG genotype for FL305DMY, FLTMY, FL305DFY, FL305DSNFY, FL305DPY were found to be 3564.74±6.0, 4584.50±6.00, 135.05±4.76, 278.53±0.06 and 113.80±0.06 respectively and for GC genotypeswere 3459.23±7.48, 4478.76±7.48, 136.19±5.96, 277.50±0.07, 112.75±0.08 respectively. Heterozygous (GC) genotype was superior for FL305DFY and GG genotype was superior for FL305DMY, FLTDMY, FL305DSNFY and FL305DPY.