Genetic variability of genetic chicken breeds estimated based on SNPS analysis in the PPARG gene

Abstract

Obesity of chickens significantly reduces the efficiency of feeding, egg production, palatability of meat carcasses, slaughter yield, consumer value, and there is also such an ecological aspect as the problem of fat utilization. The search for SNPs in genes involved in lipid metabolism is an important task today for modern research in the field of poultry farming. Differences in carcass fat between breeds and lines within a breed illustrate the importance of genetic factors in lipid metabolism in chickens. The aim of the study was to find and analyze SNPs in the PPARG gene by sequencing in various chicken breeds (n = 83). The objects of the study were chickens from the VNIIGZh bioresource collection "Genetic collection of rare and endangered chicken breeds" (Pushkin, St. Petersburg) of various productivity trends (n = 83). Blood for DNA isolation was taken from hens at the age of 330 days from a wing vein into a microtube containing 50 μl of 0.5 mM EDTA as an anticoagulant. Prior to use, blood samples were stored at -200C. Genomic DNA was isolated using a standard phenolic-detergent method. The concentration and purity of the samples were determined using a NanoDrop 2000 instrument (Thermo Fisher, USA).Analysis of the polymorphism of the regulatory region of the PPARG gene was carried out by sequencing. The primer design was carried out on the basis of information from the Internet database.The studies were carried out on the basis of the laboratory of molecular genetics of the RRIFAGB. Genetic variability for rs314476701 of the PPARG gene was determined in the analyzed breeds. SNP rs316237745 revealed a strong shift of the A.