Abstract
The occurrence of diseases is a limiting factor in the development of sorghum crop. Among the diseases that causes losses in sorghum production, anthracnose is the main and most severe, mainly by the genetic variability of the pathogen. In this context, the aim of this study was to evaluate the genetic variability of Colletotrichum sublineolum isolates. DNA were extracted from 56 monosporic isolates of C. sublineolum using a DNA extraction kit, and to perform the analysis of genetic diversity of the isolates were used ISSR primers. After amplification, it was determined the polymorphic information content (PIC), allelic frequency, UPGMA and Tocher clustering analyzes and, using software Structure, the genetic structure. According to the descriptive analysis of the genetic variability of C. Sublineolum isolates, primer AP1 presented the higher value of polymorphic information content (PIC). The higher allelic frequency was observed in loci 06, 09, 10, and 24, and the lowest in locus 02. As for the clustering method, it was observed a tendency of grouping C. sublineolum isolates according the geographic origin and, in addition to demonstrating the genetic variability between the C. sublineolum isolates, it was observed the occurrence of introgression among the isolates.
Highlights
Sorghum bicolor (L.) Moench is the world’s 5th most important cereal in relation to yield potential and planted area, being used primarily in animal feed, nutrition and biofuel
The number of band amplified per primer varied between 01 (AP3) and 08 (AP1) and AP1 showed the highest number of amplified bands, with 08 polymorphic bands, and was the most informative in demonstrating the genetic variability of C. sublineolum isolates (Table 1)
Similar results regarding the number of polymorphic bands found in this study were observed by Parreira et al (2016) when evaluated the genetic variability of C. graminicola using 15 ISSR primers, detected the presence of 66 polymorphic bands
Summary
Sorghum bicolor (L.) Moench is the world’s 5th most important cereal in relation to yield potential and planted area, being used primarily in animal feed, nutrition and biofuel. There are several phytosanitary issues that affect sorghum crop, among them are the diseases, which can cause significant losses, depending on the susceptibility of the cultivated variety to the pathogen and favorable environmental conditions to its occurrence and dissemination (Silva et al, 2008). Anthracnose is the main and the most devastating, affecting the whole plant, causing significant losses in all types of sorghum, being upper to 80% of the grain production and more than 50% of the forage (Casela et al, 2000; Pereira et al, 2011).
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