Abstract
Plaque formation with representative strains of all major influenza A virus subtypes and with influenza B (Lee) virus has been obtained in a clone (clone 1-5C-4) of a human cell line of apparently unique susceptibility. The efficiency of plaque formation relative to egg infective dose (EIU) varies with the strain of virus, ranging from 2 to 70%. The clone (1-5C-4) has remained stable with respect to viral susceptibility for at least 36 transfers in mass culture during a period of 9 months. Certain strains of virus formed plaques of characteristic and distinctive type of potential value in genetic studies. Kinetic studies of the virus-cell interaction with NWS indicated that adsorption proceeded rapidly. However, about 40% of inoculated virus was unadsorbed. Adsorption not reversed with antiserum was a slower process and required 3–4 hours. Single-cycle infection with a high input multiplicity of virus required 20 hours for completion and resulted in a yield of 4 PFU/cell.
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