Abstract
The potentials of the species of Centrosema as pasture and fodder crops in Tropical and Subtropical areas have promoted their germplasm collection and evaluation in Brazil and other countries of Central and South America. The species C. acutifolium, C. pubescens and C. brasilianum are of particular interest. Samples of the same accessions form wild materials collected in Brazil have been agronomically evaluated in Brazil and Colombia, and handled as self-pollinating species. Pairs of samples (one from Brazil and the other from Colombia) of four accessions have been genetically analyzed using isozyme markers at 16 loci. A noticeable genetic differentiation has occurred between members of each pair in few generations. Experimental evidences indicate that frequency of outcrossing are relatively high in these species. It is proposed that outcrossing between non-isolated neighboring accessions and genetic drift in small size plantings are the causes of the genetic differentiation between Brazilian and Colombian samples. Guides to evaluate and multiply Centrosema germplasm are suggested.
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