Abstract

Genetic analysis of 61 mutations of the median segment of the complex locus “14” in Podospora anserina is presented. These mutations affecting spore pigmentation have been screened following various mutagenesis (UV, Nitrosoguanidine, ICR-170) and they display several phenotypes. Comparison of complementation and genetic maps shows that this genetic region exhibits the most conspicuous genetic properties of classical operons: polarized complementation, asymmetric localization of non complementing mutants and suppressibility of some such ones. This polycistronic unit of transcription would contain five cistrons. The products coded by at least three of these genes would be involved in a multiprotein complex.

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