Abstract
All over the world, the incidence of Salmonella spp contamination on different food sources like broilers, clams and cow milk has increased rapidly in recent years. The multifaceted properties of Salomnella serovars allow the microorganism to grow and multiply in various food matrices, even under adverse conditions. Therefore, methods are needed to detect and trace this pathogen along the entire food supply network. In the present work, PFGE and ERIC-PCR were used to subtype 45 Salmonella isolates belonging to different serovars and derived from different food origins. Among these isolates, S. Enteritidis and S. Kentucky were found to be the most predominant serovars. The Discrimination Index obtained by ERIC-PCR (0.85) was slightly below the acceptable confidence value. The best discriminatory ability was observed when PFGE typing method was used alone (DI = 0.94) or combined with ERIC-PCR (DI = 0.93). A wide variety of profiles was observed between the different serovars using PFGE or/and ERIC-PCR. This diversity is particularly important when the sample origins are varied and even within the same sampling origin.
Highlights
Salmonellosis is a major health problem worldwide and accounts for high morbidity rates
Infection with Salmonella enterica occurs mainly through the consumption of contaminated food, and the estimated annual number of human infections is greater than 93.8 million cases, with 155,000 deaths per year worldwide [1]
Filter feeding organisms such as clams harvested from contaminated waters are known to concentrate high levels of Salmonella serovars leading to a high incidence of this pathogen on seafood [4]
Summary
Salmonellosis is a major health problem worldwide and accounts for high morbidity rates. The pathogenicity [3] and the increase of antimicrobial resistance in Salmonella have been recognized as the ultimate causes. Filter feeding organisms such as clams harvested from contaminated waters are known to concentrate high levels of Salmonella serovars leading to a high incidence of this pathogen on seafood [4]. This is the case in broilers [5].
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