Abstract
BackgroundPlant growth-promoting bacteria (PGPB) can be used as a safe and sustainable agricultural measure to increase plant yield. In this study, cultivable rhizospheric, endophytic, and epiphytic bacteria associated with canola were isolated on nutrient agar medium. The SDS-PAGE method was used for grouping the bacterial strains. Bacterial strains with more than 80% similarity in their protein patterns were placed in the same species and selected as a representative for partial 16S rRNA encoding gene sequence analysis.ResultsIt was found that the bacteria used in this study belonged to Pseudomonas, Frigoribacterium, Sphingomonas, Sphingobacterium, Microbacterium, Bacillus, and Rhodococcus genera. Three PGP bioactivities including siderophore production, nitrogen fixation ability, and phosphate solubilization were evaluated for 18 Pseudomonas representative strains. All tested strains were able to produce siderophore, although only 5 strains could fix nitrogen and none was able to solubilize phosphate. Greenhouse experiments showed that 4 bacterial strains (i.e., 8312, 642, 1313W, and 9421) significantly affected canola seed germination and seedling growth. Bacterial strain 8312, which was identified as Pseudomonas sp., showed the highest effect on the growth of the canola plant. These results indicated the potential of strain 8312 to be used as a biofertilizer in canola cultivation.ConclusionsThe results of this research indicated that canola-related bacteria were diverse during the flowering stage. One isolate had a significant effect on all canola plant growth factors in the greenhouse. Hence, it is recommended to investigate this strain further in field infestation experiments.
Highlights
Plant growth-promoting bacteria (PGPB) can be used as a safe and sustainable agricultural measure to increase plant yield
Isolation, screening, identification, and diversity of bacteria A total of 362 bacterial isolates associated with canola plants based on their growth on nutrient agar medium was obtained
Sodium dodecyl sulfate (SDS)-PAGE was used to screen the isolates and to prove that this method is of high efficiency to screen and group the isolates
Summary
Plant growth-promoting bacteria (PGPB) can be used as a safe and sustainable agricultural measure to increase plant yield. Many efforts have been made to find a safe and secure way to increase agricultural production In this regard, plant growth-promoting bacteria (PGPB) can be used as a safe and sustainable agricultural measure to increase plant growth and yield. Plant growth-promoting bacteria (PGPB) can be used as a safe and sustainable agricultural measure to increase plant growth and yield This method is more economical and efficient compared to chemical fertilizers while being environmentally friendly (Pathania et al 2020). PGP bacteria increase the growth and health of their host plants by different mechanisms. The plant plays a key role in determining the diversity of rhizosplane and endo-rhizosphere bacteria (Farina et al 2012)
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