Abstract
Five species of Trifolium L. (T. repens L., T. pretense L., T. hybridum L., T. campestre Schreb., and T. dubium Sibth.) were analyzed used to evaluate the genetic diversity and their phenetic relationships using inter-simple sequence repeats (ISSR) markers. Overall, T. pratense exhibited higher variation than other species. 114 amplicons were produced by ISSR markers, of which 77 (67.5%) bands were polymorphic. T. dubium showed the low genetic variation. Total genetic diversity values (HT) varied between 0.333 and 0.487, for an average over all polymorphic loci of 0.282. On a perlocus basis, the proportion of total genetic variation due to differences among species (GST) was 0.380. This indicated that about 38.0% of the total variation was among species. The estimate of gene flow, based on GST, was very low among species of genus Trifolium (Nm = 0.816). An assessment of the proportion of diversity present within species, HPOP/HSP, indicated that about 95.8% the total genetic diversity was within species. T. pratense and T. hybridum were grouped together and this clade was sister with T. repens. Two remainder species with yellow flowers were grouped together. Information on genetic diversity for Trifolium is valued for the management of germplasm and for evolving conservation strategies.Bangladesh J. Plant Taxon. 23(2): 167-173, 2016 (December)
Highlights
Trifolium L., the clover genus, is one of the largest genera in Fabaceae family
T. pratense exhibited higher variation than other species. 114 amplicons were produced by inter-simple sequence repeats (ISSR) markers, of which 77 (67.5%) bands were polymorphic
White clover is adapted to a wide climate range from the Arctic to the subtropics and has a wide altitudinal ranges
Summary
Trifolium L., the clover genus, is one of the largest genera in Fabaceae family. This genus consists about 250-300 species with a wide distribution and adaption to different agro-ecological regions (Gillet et al, 2001; Ellison et al, 2006). White clover is adapted to a wide climate range from the Arctic to the subtropics and has a wide altitudinal ranges. It is found up as 6000 m in the Himalaya Range (Baker and Williams, 1987). It has become naturalized in China, Mongolia, Korea, and Japan
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