Genetic diversity and association mapping of forage quality in diverse bermudagrass accessions

Abstract

Bermudagrass is a warm season grass widely cultivated for turf and fodder. Nonetheless, the grass has poor forage quality because animals that consume it fail to assimilate its organic matter efficiently. Thus, identification of the marker-trait association between simple sequence repeat (SSR) markers and forage-quality-related traits in diverse bermudagrass accessions would enable efficient selection of high forage quality bermudagrass cultivars. Association mapping of 8 forage-related-quality traits with 1474 markers was conducted in 60 diverse bermudagrass accessions from five geographical regions in China. Significant variations in eight phenotypic and physiological traits were observed among the 60 accessions. A total of 1474 alleles were amplified by 104 SSR primers. The average gene diversity and polymorphic information content for the study sample were 0.2097 and 0.1748 respectively. The clustering analysis suggested that geographic origin influenced genetic distances between accessions. A total of 76 markers significantly associated with traits at P < 0.01; 73 with a single trait and 3 with two traits each. Nevertheless, only 41 significant marker-trait associations (MTAs) were observed after Bonferroni test was separately conducted for each trait. Forty-one microsatellites had significant associations with 8 forage-quality-related traits. These markers provide a feasible means of genetically improving forage quality in bermudagrass after further authentication.