Abstract
Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships in genetic differentiation within 4 Chromium-treatment Leersia hexandra. The fresh leaves of Leersia hexandra cultivated on the condition of chrome pollution and exogenous organic acids were used as experimental material. The genomic DNA of Leersia hexandra was extracted by using CTAB method. The results showed that different samples of Leersia hexandra exhibited DNA polymorphism when using the random primer S43, S51and S55 as the primers in the RAPD reaction. One specific DNA band about 1000 bp was found in the sample which treated with 10 mmol/L concentration EDTA when used the S43 primer to RAPD. The obvious differences between different EDTA-treatment levels suggest that EDTA has certain effects on enrichment to heavy metals of Leersia hexandra, it will be more favored to Leersia hexandra accumulation of chromium when EDTA concentration increased.
Highlights
Leersia hexandra Swartz, a perennial marshy plant, has been reported to be a Cr-accumulating plant with high tolerance to Cr
Students concluded that DNA polymorphism detected by Random Amplified Polymorphic DNA (RAPD) analysis in conjunction with other biochemical parameters could be a powerful eco-toxicological tool in biomonitoring arsenic pollution potential [18]
Heavy metal pollution is an important form of soil pollution, the past researches focused on heavy metal toxic effect and mechanism of plants, and study on migration, accumulation, distribution and other aspects on heavy metals in the plant’s tissues, organs and ecological systems potential [19]
Summary
Leersia hexandra Swartz, a perennial marshy plant, has been reported to be a Cr-accumulating plant with high tolerance to Cr. (2014) Genetic Differentiation Caused by Chromium Treatment in Leersia hexandra Swartz Revealed by RAPD Analysis. Previous and continuous researches show that environmental factors have a significant impact on the plants [2]. Excessive Cr in soil has negative impact on plant growth. Random Amplified Polymorphic DNA (RAPD) is a kind of molecular marker based on PCR. It uses l0 bp random primers amplifying the different DNA fragments in genome to show the polymorphism [4] [5]. One RAPD amplification is a simple PCR reaction, and it suits for a large number of samples for rapid analysis. The RAPDPCR method was used to describe the pattern of DNA band variation in the samples influenced by the environmental pollution, to describe the level of pollution in an area contaminated with smoke and waste from an ironsteel factory, and to reveal the level of potential [9]
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