Abstract
Molecular confirmation of interspecific recombinants is essential to overcome the issues like self-pollination, environmental influence, and inadequacy of morphological characteristics during interspecific hybridization. The present study was conducted for genetic confirmation of mungbean (female) and mashbean (male) interspecific crosses using molecular markers. Initially, polymorphic random amplified polymorphic DNA (RAPD), universal rice primers (URP), and simple sequence repeats (SSR) markers differentiating parent genotypes were identified. Recombination in hybrids was confirmed using these polymorphic DNA markers. The NM 2006 × Mash 88 was most successful interspecific cross. Most of true recombinants confirmed by molecular markers were from this cross combination. SSR markers were efficient in detecting genetic variability and recombination with reference to specific chromosomes and particular loci. SSR (RIS) and RAPD identified variability dispersed throughout the genome. In conclusion, DNA based marker assisted selection (MAS) efficiently confirmed the interspecific recombinants. The results provided evidence that MAS can enhance the authenticity of selection in mungbean improvement program.
Highlights
PCR amplifications for random amplified polymorphic DNA (RAPD) were performed with initial denaturation at 94◦C for 4 min and 40 cycles of denaturation at 94◦C for 30 s, primer annealing at 35◦C for 1 min, primer extension at 72◦C for 2 min and final extension step at 72◦C for 7 min
Mungbean × mashbean interspecific recombinants that showed morphological characteristics similar to their respective female parents were verified as true recombinants by using RAPD, universal rice primers (URP), and simple sequence repeats (SSR) markers
Six parents (NM-92, Var.6601, NM-2006, Mash-97, Mash88, and Mash-3-156-1) involved in crossing were tested for genetic variation present at DNA level
Summary
Wilzeck) known as green gram and mashbean Happer) as black gram are widely cultivated pulse crops belonging to family Fabaceae. In cereal based cropping system, these pulses are being consumed as supplemental crops. Mungbean which is more widely cultivated as compared to mashbean has some additional properties like having more digestible proteins and low proportions of flatulence factors (Gosal and Bajaj, 1983) but are deficient in some essential amino acids compared to blackgram (Poehlman, 1991). The present investigation was extended to interspecific hybridization of green gram and black gram in order to improve the level of essential amino acids in green gram
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