Abstract
The variety of maize in Southwest Maluku (MBD) has an ability to grow in the extremely dry land, especially at Kisar island, because those areas were dominated by dry land. Dry resistance very useful to be used for genetical characterization in order to provide accurate data about the character of local maize Kisar var. Kuning Genjah. To identify the characteristics at the genome level of this local maize variety, one of the molecular methods that can be applied was RAPD molecular method. The advantage of the RAPD marker is it's technically simpler and fast in testing, does not require DNA sequence information, hence this marker becomes widely used, it only requires a small sample of DNA, the primary is commercially available and does not use radioactive compounds. The aim of this study was to find out the bands profile that has the potential to be used as a marker of dry resistance. The method applied was RAPD (Random Amplified Polymorphic DNA) using OPA-02, OPA-07, and OPC-12 primers and produces 22 DNA bands. The measurement of bands by estimating the molecular weight based on marker exponential regression. The polymorphic percentage was 90.9% between var. Kuning genjah and var. BIZI-II-Hybrid. The percentage of polymorphism showed the potential of bands that can be used as molecular markers for markers of dry resistance that can be utilized in plant breeding
Highlights
The maize varieties in Southwest Maluku (MBD)growing at the range of tolerance to the dry land, especially at Kisar Island, because the area is dominated by dry land
The RAPD marker is technically simple and fast to testing, does not require DNA sequence information, used widely, the requirement for only a small of DNA, the primary is commercially available and does not use radioactive compounds (Zulfahmi, 2013).The primers selected are often used in molecular research on maize or those that are closely related, the selection was done by using the RAPD method with a high level of polymorphism, high rates, and producing a clear band pattern
Several studies on the genetic characterization of maize had been repoted, i.e. the characteristics and kinship of local Bebo maize from Sangalla Tana Toraja, South Sulawesi with carotenoid syn 3 origin from simmyt based on molecular markers simple sequence repeat (SSR) (Siga, Juhriah, Masniawati, & Asnadi, 2015)
Summary
The maize varieties in Southwest Maluku (MBD)growing at the range of tolerance to the dry land, especially at Kisar Island, because the area is dominated by dry land. The characteristic genome level of local maize Maluku can be determine with the RAPD method (Random Amplified Polymorphic DNA). The sample used was maize Kisar (Zea mays L) var. The supernatant was removed, added 5 mL of chloroform solution: isoamilalcohol (24: 1), shaken with vortex and centrifuged again at a speed of 11.000 rpm for 10 minutes at 25oC.
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